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superresolved.com • View topic - Setting "placement.uniform.num_spots" for different submasks
Page 1 of 1

Setting "placement.uniform.num_spots" for different submasks

PostPosted: Sat Mar 29, 2014 1:08 am
by hooying
Hi,

When I run the 3B calculation on a 128-by-128-pixel image with 10-by-10-pixel submasks with a 5-pixel overlap, the reconstructed image after I piece together all submasks tends to lack contrast, or has more or less the same brightness for all features across the image. I wonder if by modifying the initial guess of the number of spots for each submask based on its averaged intensity level we can improve the overall image contrast. If so, how can we execute it since concurrent executions call a single file multispot5.cfg for initial conditions?

Thanks,
Ying

Re: Setting "placement.uniform.num_spots" for different subm

PostPosted: Wed Apr 02, 2014 8:48 pm
by edrosten

Re: Setting "placement.uniform.num_spots" for different subm

PostPosted: Sun Apr 06, 2014 4:36 am
by hooying
Thank you for the clarification.

Re: Setting "placement.uniform.num_spots" for different subm

PostPosted: Sat Feb 14, 2015 5:19 am
by desheikh
Can you provide more details of the imaging conditions and the structure that you're looking at?

Re: Setting "placement.uniform.num_spots" for different subm

PostPosted: Mon Nov 27, 2017 8:59 am
by Brunswick
Makes a lot of sense now. Thanks for the info edro.