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superresolved.com • View topic - Offset b/w Bayesian and single-emitter-fitting results

Offset b/w Bayesian and single-emitter-fitting results

Bayesian analysis of blinking and bleaching

Offset b/w Bayesian and single-emitter-fitting results

Postby hooying » Sun Apr 06, 2014 4:36 am

Hi,

I performed Bayesian analysis and single-emitter-fitting analysis using a single data file (128 by 128 px using submasks), which contains both bleach-and-blink frames and single-molecule frames. When I overlaid the two results, I found a slight offset between the two (blue: Bayesian; red: single-emitter fitting). I wonder what could have caused that. Thanks, -Ying
Attachments
Screen Shot 2014-04-05 at 9.29.59 PM.png
Screen Shot 2014-04-05 at 9.29.59 PM.png (110.99 KiB) Viewed 24786 times
hooying
 
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Re: Offset b/w Bayesian and single-emitter-fitting results

Postby edrosten » Thu Apr 10, 2014 11:31 am

edrosten
 
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Re: Offset b/w Bayesian and single-emitter-fitting results

Postby hooying » Wed Apr 16, 2014 4:21 am

Thanks Ed. It appears adding half a pixel to x and y corrected for the offset. I am attaching a screenshot with the correction.

Ying
Attachments
Screen Shot 2014-04-15 at 9.20.10 PM.png
Screen Shot 2014-04-15 at 9.20.10 PM.png (72.3 KiB) Viewed 24764 times
hooying
 
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Re: Offset b/w Bayesian and single-emitter-fitting results

Postby hooying » Wed Apr 16, 2014 11:03 pm

Is it possible the offset is 1 instead of 0.5 pixel? I am attaching a picture with the Bayesian result offset by one pixel and the alignment appears to be better with the single-emitter fitting data. The x and y axis is labeled by the pixel number and the pixel size is 160 nm. Ying
Attachments
Screen Shot 2014-04-16 at 3.57.05 PM.png
Screen Shot 2014-04-16 at 3.57.05 PM.png (116.46 KiB) Viewed 24763 times
hooying
 
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Re: Offset b/w Bayesian and single-emitter-fitting results

Postby edrosten » Fri Apr 18, 2014 3:42 pm

edrosten
 
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Re: Offset b/w Bayesian and single-emitter-fitting results

Postby FrankGarza » Wed Dec 31, 2014 7:22 am

It's hard to state with out more information. Have the level club to the photograph?
One of the most typical result in with regard to offsets using these kinds of facts differs events regarding coordinates: it really is human judgements if thez centre or even corner of any pixel is at (0, 0). It will produce the diagonal balanced out precisely 50 % the pixel. Will of which reveal the particular balanced out, or even may of which not fit?
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