Why 3B uses all coordinates of every iteration for reconstr?

Bayesian analysis of blinking and bleaching

Why 3B uses all coordinates of every iteration for reconstr?

Hi,

We are trying reconstructing some images and we have looked that you take all the coordinates of the result file, no matter the iteration in which they were computed. Our question is that if the model is being refined in every iteration why not just take from the result file the coordinates computed in the latest iteration?

Looking forward...
Haydee O. Hdez. Aviña
Instituto de Biotecnología
hoha

Posts: 9
Joined: Mon Apr 14, 2014 9:24 pm

Re: Why 3B uses all coordinates of every iteration for recon

hoha wrote:Our question is that if the model is being refined in every iteration why not just take from the result file the coordinates computed in the latest iteration?

This is essentially because we are using a Bayesian approach rather than a maximum likelihood fit. Our algorithm is a sampling algorithm is the latest iteration is not necessarily better than the previous iteration, it's a different sampling instead. There is a notion of convergence, but this is a probabilistic convergence to drawing points from the same distribution, rather than convergence to a local maximum. It's the MCMC sampling of the fluorophore states which makes the result of the fitting stage noisy.

The trouble is that the data is too noisy and ambiguous to reliably perform a maximum likelihood fit.
edrosten

Posts: 42
Joined: Wed Jul 18, 2012 2:54 pm

Re: Why 3B uses all coordinates of every iteration for recon

Hi edrosten,

Thank you very much. These information are really helpful.

Thanks.
hareem

Posts: 1
Joined: Thu Jan 08, 2015 7:08 am