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superresolved.com • View topic - Role FWHM and Pixel Size during the image reconstruction

Role FWHM and Pixel Size during the image reconstruction

Bayesian analysis of blinking and bleaching

Role FWHM and Pixel Size during the image reconstruction

Postby william2014 » Wed Dec 17, 2014 2:51 pm

Hi there,

i have 3 questions concerning the influence of the blur FWHM and pixel Size during the image reconstruction with 3B using the imageJ plugin:

- What are the role of the blur FWHM and pixel size during the reconstruction? If i change them at any stage of the reconstruction, can this affect my reconstruction?
- At the beginning of the programm, how should i set the Blur FHWM and the pixel size, if i want to obtain a very good image reconstruction ( in the 3b help Tutorial, blur=16.1 nm and pixel= 3.0 nm).
- If the Blur FHWM and the number of Pixel are small, the programm will run slow of fast?

Thanks
william2014
 
Posts: 3
Joined: Thu Nov 13, 2014 10:20 am

Re: Role FWHM and Pixel Size during the image reconstruction

Postby edrosten » Thu Dec 18, 2014 5:40 pm

edrosten
 
Posts: 42
Joined: Wed Jul 18, 2012 2:54 pm


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