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In the multispot5.cfg file

PostPosted: Wed Jan 14, 2015 12:23 pm
by faarizamar
In the multispot5.cfg file, we were supplied with three sets of values for blur.mu and blur.sigma according to the pixel size (100, 160 and 79nm per pixel). For pixel sizes not listed here (from custom setups), are we Okay by using values from the pixel size closest to the actual value? Alternatively, how can we determine these values based on the pixel size?

Re: In the multispot5.cfg file

PostPosted: Fri Jan 23, 2015 11:00 am
by edrosten
faarizamar wrote:In the multispot5.cfg file, we were supplied with three sets of values for blur.mu and blur.sigma according to the pixel size (100, 160 and 79nm per pixel). For pixel sizes not listed here (from custom setups), are we Okay by using values from the pixel size closest to the actual value? Alternatively, how can we determine these values based on the pixel size?


It's best to use the correct settings. There are some instructions and forumlae for calculating the right values in this post:

viewtopic.php?f=10&t=30209


Regards

-Ed