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superresolved.com • View topic - Setting "placement.uniform.num_spots" for different submasks

Setting "placement.uniform.num_spots" for different submasks

Bayesian analysis of blinking and bleaching

Setting "placement.uniform.num_spots" for different submasks

Postby hooying » Sat Mar 29, 2014 1:08 am

Hi,

When I run the 3B calculation on a 128-by-128-pixel image with 10-by-10-pixel submasks with a 5-pixel overlap, the reconstructed image after I piece together all submasks tends to lack contrast, or has more or less the same brightness for all features across the image. I wonder if by modifying the initial guess of the number of spots for each submask based on its averaged intensity level we can improve the overall image contrast. If so, how can we execute it since concurrent executions call a single file multispot5.cfg for initial conditions?

Thanks,
Ying
hooying
 
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Re: Setting "placement.uniform.num_spots" for different subm

Postby edrosten » Wed Apr 02, 2014 8:48 pm

edrosten
 
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Re: Setting "placement.uniform.num_spots" for different subm

Postby hooying » Sun Apr 06, 2014 4:36 am

Thank you for the clarification.
hooying
 
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Re: Setting "placement.uniform.num_spots" for different subm

Postby desheikh » Sat Feb 14, 2015 5:19 am

Can you provide more details of the imaging conditions and the structure that you're looking at?
desheikh
 
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Re: Setting "placement.uniform.num_spots" for different subm

Postby Brunswick » Mon Nov 27, 2017 8:59 am

Makes a lot of sense now. Thanks for the info edro.
Brunswick
 
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