Setting "placement.uniform.num_spots" for different submasks

[hooying]

Hi,

When I run the 3B calculation on a 128-by-128-pixel image with 10-by-10-pixel submasks with a 5-pixel overlap, the reconstructed image after I piece together all submasks tends to lack contrast, or has more or less the same brightness for all features across the image. I wonder if by modifying the initial guess of the number of spots for each submask based on its averaged intensity level we can improve the overall image contrast. If so, how can we execute it since concurrent executions call a single file multispot5.cfg for initial conditions?

Thanks,
Ying

[edrosten]

[hooying]

Thank you for the clarification.

[desheikh]

Can you provide more details of the imaging conditions and the structure that you're looking at?

[Brunswick]

Makes a lot of sense now. Thanks for the info edro.